A3311
Bovine Serum Albumin
powder, BioXtra
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
Product Name
Bovine Serum Albumin, powder, BioXtra
loss
≤5%
pH
6.5-7.5
biological source
bovine
product line
BioXtra
assay
≥98% (agarose gel electrophoresis)
form
powder
mol wt
~66 kDa
packaging
poly bottle of
origin
USA origin
technique(s)
cell culture | embryo: suitable
solubility
water: soluble (40 mg/ml)
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.
Biochem/physiol Actions
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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