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A7420

Amyloglucosidase from Aspergillus niger

greener alternative

lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

Synonym(s):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS Number:
9032-08-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-877-2
MDL number:
MFCD00081350
EC Number:
3.2.1.3 (BRENDA, IUBMB)

Key Documents

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Product Name

Amyloglucosidase from Aspergillus niger, lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose

form

lyophilized powder

specific activity

30-60 units/mg protein (biuret)

composition

protein, ≥80%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

impurities

≤0.02% glucose

greener alternative category

, Enabling

storage temp.

−20°C

Quality Level

200

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Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Physical form

Lyophilized powder containing less than 0.02% glucose

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH6247
SLCK0132
SLCF0968
SLCD5495
SLBX1747

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Product A7420,  Amyloglucosidase from Aspergillus niger?

    Amyloglucosidase from A. niger exists in two isoforms with MWs of 69,810 and 89,130, as determined by SDS-PAGE. See: Starch, 34, 346 (1982).

  6. Does Product A7420,  Amyloglucosidase from Aspergillus niger, contain any stabilizers or additives?

    The starting material for Product No. A7420 is Product No. A3042, which is a preparation of the enzyme in a 1 M glucose solution. This is dialyzed against water until the glucose concentration is less than 0.02%, and then lyophilized.

  7. How can a solution of Product A7420,  Amyloglucosidase from Aspergillus niger, be prepared?

    A 1 mg/mL solution may be prepared in water.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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