B0300
Betaine solution
5 M, PCR Reagent
Synonym(s):
TMG, trimethylglycine
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About This Item
Linear Formula:
(CH3)3N+CH2COO−
CAS Number:
Molecular Weight:
117.15
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3537113
Product Name
Betaine solution, 5 M, PCR Reagent
InChI key
KWIUHFFTVRNATP-UHFFFAOYSA-N
InChI
1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3
SMILES string
C[N+](C)(C)CC([O-])=O
form
liquid
packaging
vial of 1.5 mL
concentration
5 M
technique(s)
PCR: suitable
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
2-8°C
Quality Level
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Application
Betaine solution has been used as a component of the reaction mixture for:
- loop-mediated isothermal amplification (LAMP) reactions
- polymerase chain reaction for genomic DNA amplification
- quantitative polymerase chain reaction (qPCR) for telomere length analysis
- in the PCR amplification of CGG repeats in the genomic DNA
- reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
- PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)
- reverse transcription during library preparation using Smartseq2
General description
Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.
Packaging
1.5mL per vial
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards
Kostic T, et al.
Applied Microbiology and Biotechnology, 99(18), 7711-7722 (2015)
Molecular characterization of X chromosome fragility in idiopathic mental retardation
Omar HAH, et al.
The Egyptian journal of medical human genetics., 17(2), 165-172 (2016)
DMSO and betaine greatly improve amplification of GC-rich constructs in de novo synthesis
Jensen, Michael A and Fukushima, Marilyn and Davis, Ronald W
PLoS ONE, 5(6), e11024-e11024 (2010)
P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and
Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)
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