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Merck

D1878

2,6-Dichloroindophenol sodium salt hydrate

Powder, BioReagent

Synonym(s):

2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP

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About This Item

Empirical Formula (Hill Notation):
C12H6Cl2NNaO2 · xH2O
CAS Number:
1266615-56-8
Molecular Weight:
290.08 (anhydrous basis)
Beilstein/REAXYS Number:
3641229
NACRES:
NA.47
PubChem Substance ID:
57654075
UNSPSC Code:
12171500
EC Number:
210-640-4
MDL number:
MFCD00150014

Key Documents

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Product Name

2,6-Dichloroindophenol sodium salt hydrate, suitable for vitamin C determination, BioReagent

InChI key

XHSOLXWCGCVQHE-UHFFFAOYSA-M

InChI

1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1

SMILES string

O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2

product line

BioReagent

form

powder

solubility

H2O: soluble 10 mg/mL, clear, blue to very deep blue

suitability

suitable for vitamin C determination

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.

Application

2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
  • As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
  • To compose UVB specific dosimeter.
  • Quantification of vitamin C (L-ascorbic acid) in fruit juices.
  • As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.
  • Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Andrew Mills et al.
The Analyst, 134(5), 845-850 (2009-04-22)
A UVB specific dosimeter is described comprising: a redox dye (2,6-dichloroindophenol, DCIP), a semiconductor (tin(IV) oxide, SnO(2)) and a sacrificial electron donor (glycerol) dispersed in a polymer (hydroxy ethyl cellulose, HEC) film. The dosimeter is blue in the absence of
Spectrophotometric evaluation of gold nanoparticles as red-ox mediator for glucose oxidase.
Ramanaviciene A, et al.
Sensors and Actuators B, Chemical, 137(2), 483-489 (2009)
Further insights toward vitamin c determination and stability proposal of a new quantification method
Vieira CA, et al.
Biosci., 26(2), 296-304 (2010)
Hanit Brenner-Lavie et al.
Biochimica et biophysica acta, 1777(2), 173-185 (2007-11-13)
Deleterious effects of dopamine (DA) involving mitochondrial dysfunction have an important role in DA-associated neuronal disorders, including schizophrenia and Parkinson's disease. DA detrimental effects have been attributed to its ability to be auto-oxidized to toxic reactive oxygen species. Since, unlike
Misty Prince et al.
Toxicology letters, 185(3), 180-186 (2009-01-20)
Naturally occurring coumarins possess anti-carcinogenic activities in part by inducing carcinogen-detoxifying enzymes glutathione S-transferase (GST) and/or NAD(P)H quinone oxidoreductase (NQO1). Our goal was to determine whether citrus coumarins induce hepatic GST and/or NQO1 via activation of Nrf2 and the antioxidant
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