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Merck

F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
EC Number:
289-149-2
MDL number:
MFCD00131062

Key Documents

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Product Name

Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture

Quality Level

200

biological source

human foreskin fibroblasts

product line

BioReagent

form

lyophilized powder

mol wt

~550 kDa

packaging

pkg of 0.5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

impurities

Human Source, none detected (HCV)
 Human Source, none detected (HIV)
 Human Source, none detected (HTLV)
 Human Source, none detected (HbSAg)
 Microbial Contamination, passes test

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_212482.4

UniProt accession no.

P02751

binding specificity

Peptide Source: Collagen

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... FN1(2335)

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Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells
Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Disclaimer

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Features and Benefits

  • Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
  • It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
  • The starting material is tested and found negative for HIV and HBsAg.
  • The product is soluble in sterile water, yielding a clear, colorless solution.
  • It can be stored at -20°C for at least three years.

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

Store reconstituted solution in working aliquots at -20°C or lower.
dialyzed against chloroform

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
049M4164V
11180632
087M4132V
11170515
037M4075V

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Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery.
Tsang T M, et al.
Infection and Immunity, 78(8), 3358-3368 (2010)
Ursula Fürnkranz et al.
Acta veterinaria Scandinavica, 55, 63-63 (2013-09-10)
The cell invasiveness of Mycoplasma gallisepticum, the causative agent of respiratory disease in chickens and infectious sinusitis in turkeys, may be a substantial factor in the well-known chronicity of these diseases and in the systemic spread of infection. To date
Regulation of matrix Accumulation (2012)
Caroline Chagnot et al.
PloS one, 8(3), e59386-e59386 (2013-03-22)
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 are responsible for repeated food-poisoning cases often caused by contaminated burgers. EHEC infection is predominantly a pediatric illness, which can lead to life-threatening diseases. Ruminants are the main natural reservoir for EHEC and food contamination
Natural variant of collagen-like protein A in serotype M3 group A Streptococcus increases adherence and decreases invasive potential.
Flores A R, et al.
Infection and Immunity (2015)
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