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G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

Synonym(s):

Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH

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About This Item

CAS Number:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.49 (BRENDA, IUBMB)
MDL number:
MFCD00081656
Specific activity:
≥550 units/mg protein (biuret)
Biological source:
bacterial (Leuconostoc mesenteroides)
Recombinant:
expressed in E. coli

Key Documents

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Product Name

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

biological source

bacterial (Leuconostoc mesenteroides)

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

UPI0021A4D550

foreign activity

creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%

storage temp.

2-8°C

Quality Level

300

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Application

Glucose-6-phosphate dehydrogenase has been used:

  • to test ketose reductase activity in developing maize endosperm.
  • to determine the levels of mannose in coronary heart disease patient-derived serum
  • to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication
  • to determine the glucose uptake in cultured human muscle satellite cells

Biochem/physiol Actions

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).G6PD is a dimer and consists of a single active site at each subunit.

Research area: Cell Signaling

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

Physical form

Suspension in 3.2 M (NH4)2SO4 containing 50 mM Tris and 1 mM MgCl2, pH 7.5

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
079M4054V
50190301
128M4011V
SLBS8444
078M4008V

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Cara A Griffiths et al.
Nature, 540(7634), 574-578 (2016-12-16)
The pressing global issue of food insecurity due to population growth, diminishing land and variable climate can only be addressed in agriculture by improving both maximum crop yield potential and resilience. Genetic modification is one potential solution, but has yet
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Mareike Hauenstein et al.
Bio-protocol, 7(18), e2561-e2561 (2017-09-20)
Hydroxylation of chlorophyll catabolites at the so-called C32 position ( Hauenstein et al., 2016 ) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
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