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Merck

H0262

HAT Media Supplement (50×) Hybri-Max™

lyophilized powder, suitable for hybridoma, BioXtra

Synonym(s):

HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Product Name

HAT Media Supplement (50×) Hybri-Max™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

HAT Media Supplement (50×) Hybri-Max™ has been used:
  • in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
  • in the generation of mAbs (monoclonal antibodies)
  • in the luciferase-based bioassay (LUC) assay

Preparation Note

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK9096
SLCK1608
SLCK1606
SLCK2064
SLCK0063

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Geoffrey A Mueller et al.
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The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of immunology (Baltimore, Md. : 1950), jimmunol-j0804281 (2009)
The expression and function of the NKRP1 receptor family in C57BL/6 mice
Aust J G, et al.
Journal of Immunology, jimmunol-j0804281 (2009)
Youngkyun Kim et al.
Clinical & translational immunology, 4(11), e51-e51 (2015-12-19)
This study was undertaken to develop a novel anti-citrullinated peptide antibody (ACPA) and to investigate its arthritogenicity in a collagen-induced arthritis (CIA) model. The novel ACPA, 12G1, was developed by injecting cyclic citrullinated antigen in mice and subsequently hybridizing the
Development and validation of cell-based luciferase reporter gene assays for measuring neutralizing anti-drug antibodies against interferon beta
Hermanrud C, et al.
Journal of Immunological Methods, 1-9 (2016)
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