L1667
Lysozyme human
Lysobac™, recombinant, expressed in rice, lyophilized powder, ≥100,000 units/mg protein
Synonym(s):
Lysobac™ r Lysozyme, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Lysozyme human, Lysobac™, recombinant, expressed in rice, lyophilized powder, ≥100,000 units/mg protein
biological source
human
recombinant
expressed in rice
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥100,000 units/mg protein
mol wt
16.5 kDa
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... LYZ(4069)
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Application
Lysozyme human has been used:
- in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
- in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
- in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)
Biochem/physiol Actions
Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.
Features and Benefits
Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:
Advantages:
- Improves Bacterial Fermentation Efficiency
- Greater bacterial lysis activity per mg.
- 1 gram of delivers more lysis activity than 4 grams of HEWL
- Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
- Improved recombinant protein yield compared to mechanical lysis
- Reduces cost of goods
General description
Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.
Legal Information
rhLysozyme manufactured in the USA by InVitria.
Lysobac is a trademark of InVitria
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Packaging
Package size based on protein content
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Thermodynamics and binding mechanism of polyphenon-60 with human lysozyme elucidated by calorimetric and spectroscopic techniques
Yasmeen S, et al.
The Journal of Chemical Thermodynamics, 110(5), 79-86 (2017)
A novel lysozyme mutation Phe57Ile associated with hereditary renal amyloidosis
Yazaki M, et al.
Kidney International, 63(5), 1652-1657 (2003)
Suppression of human arthritis synovial fibroblasts inflammation using dexamethasone-carbon nanotubes via increasing caveolin-dependent endocytosis and recovering mitochondrial membrane potential
Lee YK, et al.
International journal of nanomedicine, 12(5), 5761-5761 (2017)
Inflammatory bowel disease: an impaired barrier disease
Jager S, et al.
Langenbeck'S Archives of Surgery / Deutsche Gesellschaft Fur Chirurgie, 398(1), 1-12 (2013)
Expression of natural antimicrobial human lysozyme in rice grains
Huang J, et al.
Molecular breeding : new strategies in plant improvement, 10(1-2), 83-94 (2002)
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