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Merck

M0404

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym(s):

MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72
MDL number:
MFCD00240976

Key Documents

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Product Name

Murashige and Skoog Basal Medium, powder, suitable for plant cell culture, with Gamborg′s vitamins

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

Quality Level

200

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies
  • in the suspension culture of Nicotiana plumbaginifolia for transformation
  • in the root culture of hairy clone A. artemisiifolia T4

Preparation Note

Formulated to contain 4.4 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

10043-35-3

CAS No.

6484-52-2

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN4595
SLCN4595
SLCM2481
SLCK9071
SLCK3342

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Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Gilson PR, et al.
Planta, 219(6), 1050-1056 (2004)
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Pseudomonas syringae subverts plant immune signalling through injection of type III secreted effectors (T3SE) into host cells. The T3SE HopF2 can disable Arabidopsis immunity through Its ADP-ribosyltransferase activity. Proteomic analysis of HopF2 interacting proteins identified a protein complex containing ATPases
Statistical analysis of elicitation strategies for thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia
Bhagwath SG and Hjortso MA
Journal of Biotechnology, 80(2), 159-167 (2000)
John E McLaughlin et al.
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