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Merck

M1018

Minimum Essential Medium Eagle

With Hanks′ salts, ʟ-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

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Product Name

Minimum Essential Medium Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Preparation Note

Formulated to contain 10.7 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH5166
SLCH5310
SLCG8527
SLBX1562
SLBQ5816V

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