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Merck

M3058

MES sodium salt

BioPerformance Certified, suitable for cell culture

Synonym(s):

4-Morpholineethanesulfonic acid, sodium salt, Sodium 2-Morpholinoethanesulfonate, 2-(N-Morpholino)ethanesulfonic acid sodium salt, 4-Morpholineethanesulfonic acid sodium salt

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About This Item

Empirical Formula (Hill Notation):
C6H12NNaO4S
CAS Number:
71119-23-8
Molecular Weight:
217.22
NACRES:
NA.25
PubChem Substance ID:
24896792
eCl@ss:
32129211
UNSPSC Code:
12161700
EC Number:
275-203-2
MDL number:
MFCD00065473
Beilstein/REAXYS Number:
3765682

Key Documents

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InChI key

IRHWMYKYLWNHTL-UHFFFAOYSA-M

InChI

1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1

SMILES string

[Na+].[O-]S(=O)(=O)CCN1CCOCC1

grade

BioPerformance Certified, Molecular Biology

form

crystalline powder

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

useful pH range

5.5-6.7

pKa 

6.1

solubility

water: 335.3 g/L at 20 °C

suitability

suitable for component for culture media, suitable for molecular biology

application(s)

agriculture
diagnostic assay manufacturing
life science and biopharma
sample preparation

foreign activity

DNase, NICKase, RNase, protease, none detected

Quality Level

400

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General description

4-Morpholineethanesulfonic acid, sodium salt (MES) is a popular zwitterionic buffer in scientific research due to its excellent properties. It can buffer solutions effectively within a pH range of 5.5-6.5 with a pKa value of 6.1. This product is BioPerformance certified and it finds extensive application as a buffering agent in biochemistry and molecular biology experiments, contributing to the stabilization of pH in different biological reactions including western blotting, cell culture and electrophoresis.
MES is one of the so-called "Good" buffers developed for biological applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Application

MES sodium salt has been employed as a coupling buffer for amine-reactive sensors at either pH 4.5 or pH 5.0 to facilitate the coupling of monoclonal antibodies (MAbs) onto the sensors, used in hybridization studies with fluorescently-labeled complementary strands and microarray slide washing during microarray analysis, and played a role in preparing a new set of RNA phosphoramidites compatible with in situ photolithography for growing RNA oligonucleotides on microarrays.
MES sodium salt has been used as a coupling buffer during the coupling of MAbs onto amine-reactive sensors. It has also been used as a component of germination medium and shoot induction medium during in vitro plant regeneration.

Features and Benefits

  • Suitable for Molecular Biology and Cell Culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, and Protease
  • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions are stable at 2-8°C for months. Sterilization should be done by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCB4597
SLCB4597
SLCK8073
SLCK8629
SLCJ6454

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In vitro plant regeneration from commercial cultivars of soybean.
Raza, G
Biomedical Research, 2017 (2017)
High-throughput epitope binning assays on label-free array-based biosensors can yield exquisite epitope discrimination that facilitates the selection of monoclonal antibodies with functional activity.
Abdiche, YN et al.
PLoS ONE, 9 (3), e92451-e92451 (2014)
Dawson, RMC et al.
Data for Biochemical Research, 410-410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)
Elisabet Fuguet et al.
Electrophoresis, 29(13), 2841-2851 (2008-06-12)
The performance of the most common and also some other less common CE buffers has been tested for the pKa determination of several types of compounds (pyridine, amines, and phenols). The selected buffers cover a pH ranging from 3.7 to
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