M4530
Medium 199
With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
Medium 199 has been used:
- as a suspension media to maintain the primary neuronal cultures
- as a component of the collagen gel for ex vivo embryonic heart culture
- to maintain the ovarian tissue sections as part of the slow-freezing protocol
General description
Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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