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Merck

NA0200

GenElute™ HP Plasmid Midiprep Kit

greener alternative

sufficient for 25 purifications

Synonym(s):

GenElute™ HP Plasmid Midiprep Kit, HP midiprep kit, fast midiprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501

Key Documents

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Product Name

GenElute™ HP Plasmid Midiprep Kit, sufficient for 25 purifications

usage

sufficient for 25 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

, Aligned

storage temp.

15-25°C

Quality Level

200

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Application

GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required
  • Contains fewer plastic components, reducing the amount of waste

General description

The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Click here to view the kit protocol

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL6105
SLCL6104
SLCJ9943
SLCK8455
SLCK8453

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