NA1020
GenElute™ PCR Clean-Up Kit
sufficient for 70 purifications
Synonym(s):
Gen Elute, PCR Purification
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106300
usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
Quality Level
input
DNA (single or double stranded PCR ampliflied)
test parameters
sample volume: 10 μg, sample volume: 100 μL
storage temp.
15-25°C
Related Categories
General description
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp) are removed.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Application
GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Biochem/physiol Actions
The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).
Features and Benefits
- Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Removes over 99% of primers and other components
- Eliminates the need to remove mineral oil by organic extraction
- 40% more purification preps supplied than market leader
- Recovers up to 95% of PCR products between 100 bp and 10 kb
- Purifies up to 100 μL or 10 μg of PCR amplified DNA in 8 minutes
- Removes over 99% of primers and other components
Other Notes
The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
Storage Class
10 - Combustible liquids
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