P7208
Pertussis toxin
from Bordetella pertussis, lyophilized powder, protein endotoxin
Synonym(s):
Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
Product Name
Pertussis toxin from Bordetella pertussis, lyophilized powder
SMILES string
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
InChI key
WOPKHAQDUMDJIY-UHFFFAOYSA-N
form
lyophilized powder
storage temp.
2-8°C
Quality Level
Gene Information
Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)
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Application
Inactive toxin form released from B. pertussis.
Pertussis toxin from Bordetella pertussis has been used: as a blocker of the Gαi subunit of the chemokine (CXCR4) receptor to measure the intracellular Ca2+ in breast cancer cell lines, to characterize the Gi signaling involved in ADP-induced tissue factor (TF) and P-selectin exposure, to induce autoimmune vasculitis in a rat model
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Biochem/physiol Actions
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
This compound is featured on the G Proteins (Heterotrimeric) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
General description
Pertussis toxin (PT) is a multi-subunit complex toxin that possesses one active subunit(A) and five binding subunits (B5). PT enters the mammalian cells via endocytosis by attaching itself to the glycosylated molecules on the surface of these cells.
Physical form
Lyophilized powder containing sodium chloride and sodium phosphate buffer salts
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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