S8761
Sodium Bicarbonate
Solution (7.5%), sterile-filtered, suitable for cell culture
Synonym(s):
aqueous sodium bicarbonate
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About This Item
CAS Number:
UNSPSC Code:
12161702
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Product Name
Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
sterility
sterile-filtered
product line
BioReagent
form
solution (7.5%)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.8-8.2
storage temp.
2-8°C
Quality Level
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Application
Sodium bicarbonate solution has been used:
- as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
- as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
- as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
- to prepare collagen mix droplets for 3D collagen sprouting assay
- to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture
General description
Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.
Preparation Note
Prepared in cell culture grade water.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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