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WGA4

GenomePlex® Single Cell Whole Genome Amplification Kit

Amplify genome of a single cell

Synonym(s):

Single cell whole genome amplification, Whole genome amplification

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About This Item

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55

Key Documents

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Product Name

GenomePlex® Single Cell Whole Genome Amplification Kit, Amplify genome of a single cell

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Related Categories

Application

GenomePlex® Single Cell Whole Genome Amplification Kit has been used:

  • to amplify the isolated DNA
  • in whole genome amplification (WGA)
  • to amplify the microdissected DNAs
  • for the amplification of circulating tumor cell genomic DNA from metastatic castration-resistant prostate cancer cells
  • for the detection of copy number variations (CNV) by single-cell low-coverage whole-genome sequencing (SLWGS) method
  • Gel electrophoresis
  • qPCR
  • comparative genomic hybridization (CGH) microarray
  • Short tandem repeats (STR) analysis
  • single nucleotide polymorphism (SNP) analysis

Features and Benefits

  • Highly yield and accuracy of DNA amplification within four hours
  • Amplification of DNA from any source such as cancer cells, epithelial cells, lymphocytes, fibroblast amniotic cells, polycarbonate fixed cells, and plant cells
  • A complete representation of the entire genome with minimal allele bias
  • WGA DNA polymerase is suitable for use with downstream applications including gel electrophoresis, qPCR, comparative genomic hybridization (CGH) microarray, short term repeat (STR) analysis, and single nucleotide polymorphism (SNP) analysis, TaqMan® assays, and microsatellite analysis

General description

GenomePlex® Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. This kit is designed to amplify the genome of a single cell. This rapid and straightforward method provides millionfold amplification yielding microgram quantities of genomic DNA from a single cell. Traditional single-cell whole genome amplification methods yield insufficient quantities with significantly biased representation. The kit includes all the reagents necessary for cell lysis and successive whole genome amplification. Single cells can be isolated by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), dilution, or any other applicable method.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCL2172
SLCK0379
SLCK6880
SLCJ8960
SLCF6655

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can the amplified Omniplex products resulting from the use of Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used for TA cloning?

    Yes.  Please ensure that a final extension of 7-30 minutes at 72°C is included after amplification if performing subsequent TA cloning reactions.

  4. Can Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used with purified DNA?

    Yes. The kit is designed to amplify the small amount of DNA that is present in a single cell (3-5 pg). It will also work with small amounts of purified DNA; we recommend less than 100 pg.

  5. What kinds of cells can be used as starting materials with Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit?

    The cell lysis method involves treatment with proteinase K and heat. Most cells will be lysed under these conditions.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nathan R Treff et al.
Molecular human reproduction, 16(8), 583-589 (2010-05-21)
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained
Resolving tumor heterogeneity: genes involved in chordoma cell development identified by low-template analysis of morphologically distinct cells
El-Heliebi A, et al.
PLoS ONE, 9(2), e87663-e87663 (2014)
Heike Fiegler et al.
Nucleic acids research, 35(3), e15-e15 (2006-12-21)
Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount
Jochen B Geigl et al.
Nucleic acids research, 37(15), e105-e105 (2009-06-23)
Clinical DNA is often available in limited quantities requiring whole-genome amplification for subsequent genome-wide assessment of copy-number variation (CNV) by array-CGH. In pre-implantation diagnosis and analysis of micrometastases, even merely single cells are available for analysis. However, procedures allowing high-resolution
View All Related Papers

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