3050-OP
CHAPS
OmniPur® Grade
Synonym(s):
CHAPS, 3-[(3-Cholamidopropyl)-dimethylammino]-propane- sulfonate, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
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About This Item
Product Name
CHAPS, OmniPur® Grade, Zwitterionic detergent
InChI
1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES string
C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI key
UMCMPZBLKLEWAF-RFCNGIAKSA-N
form
crystalline powder
mol wt
micellar avg mol wt 6150
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
aggregation number
10
color
white
CMC
6 mM (20-25°C)
transition temp
cloud point >100 °C
foreign activity
DNAse, none detected, Protease, none detected, RNAse, none detected
shipped in
ambient
storage temp.
10-30°C
Quality Level
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General description
Suitable for Molecular Biology
Conductivity (10%, water): ≤50 µmho
DNase: None detected
Protease: None detected
Purity (TLC): Single spot
RNase: None detected
Solubility: 20% in water, passes test
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Application
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 3050CN | 04055977230048 |
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