01494
Nancy-520
suitable for RT-PCR, ≥90.0% (HPCE)
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About This Item
UNSPSC Code:
12171500
EC Number:
200-664-3
NACRES:
NA.32
Quality Segment
assay
≥90.0% (HPCE)
form
liquid
mol wt
78.13 g/mol
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
color
red to very dark red
bp
189 °C/1,013 hPa
mp
18.4 °C
solubility
DMSO: soluble
density
1.104 g/cm3 at 20 °C
fluorescence
λex 520 nm; λem 554 nm in TE buffer; DNA
greener alternative category
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry
Application
Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520
Greener alternative to Ethidium bromide.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520
Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520
Greener alternative to Ethidium bromide.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup - Solvent
flash_point_c
87 °C - closed cup - Solvent
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Saunders, N.A. and Lee, M.A.
Real-Time PCR: Advanced Technologies and Applications (2013)
van Pelt-Verkuil, E., van Belkum, A. and Hays, J.P
Principles and Technical Aspects of PCR Amplification (2008)
Rodriguez-Lazaro, D.
Real-Time PCR in Food Science: Current Technology and Applications (2013)