C-12380
Human Aortic Adventitial Fibroblasts (HAoAF)
500,000 cryopreserved cells
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About This Item
NACRES:
NA.81
UNSPSC Code:
41106514
eCl@ss:
32160703
Biological source:
human aorta
Morphology:
(fibroblast)
Relevant disease(s):
hypertension; cardiovascular diseases
biological source
human aorta
product line
PromoCell®
packaging
pkg of 500,000 cells
morphology
(fibroblast)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
hypertension; cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Application
Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue and are provided in a cryopreserved format. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.
Biochem/physiol Actions
Aorta
Preparation Note
Click here for more information.
Analysis Note
Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Other Notes
Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positiveGuaranteed population doublings: >10
Recommended Primary Cell Culture Media:Link
Legal Information
PromoCell is a registered trademark of PromoCell GmbH
Disclaimer
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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