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About This Item
UNSPSC Code:
41106514
eCl@ss:
32160903
Biological source:
human kidney (fetal)
Growth mode:
adherent
Morphology:
small slightly rounded cells growing in a dispersed manner
Manufacturer/tradename:
ECACC
Product Name
293T cell line human, NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth
biological source
human kidney (fetal)
description
In Australia, this cell line is only supplied as live culture in flask due to the challenging recovery. Please store at 37°C upon receipt., Shipping condition: Ambient (Australia)
packaging
pkg of vial of cells 12022001-1VL, tube of 5 μg 12022001-DNA-5UG
manufacturer/tradename
ECACC
growth mode
adherent
morphology
small slightly rounded cells growing in a dispersed manner
products
SV40 T antigen
technique(s)
cell culture: suitable using | mammalian
shipped in
dry ice
storage temp.
−196°C
General description
Application
293T cell line human has been used:
- for lentiviral particle production
- in the expression of eukaryotic proteins
- for small interfering RNA (siRNA) transfection
- for human immunodeficiency virus (HIV) transfection for pseudoviruses production
- as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression
Biochem/physiol Actions
Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.
STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19
The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.
Packaging
NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.
Preparation Note
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2.
Other Notes
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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