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About This Item
UNSPSC Code:
41106514
Biological source:
human lung (fetal)
Growth mode:
Adherent
Karyotype:
Modal no. = 60-80, hyperdiploid
Morphology:
Fibroblast
Manufacturer/tradename:
ECACC
Product Name
MRC-5 SV2, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84100401, human lung (fetal), Fibroblast
biological source
human lung (fetal)
packaging
pkg of vial of cells 84100401-1VL, tube of 5 μg 84100401-DNA-5UG
manufacturer/tradename
ECACC
growth mode
Adherent
karyotype
Modal no. = 60-80, hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Transformation and virus studies: SV40
Biochem/physiol Actions
Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.
Human foetal lung, SV40 transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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