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D2650

Dimethyl sulfoxide

Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Synonym(s):

DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
24893703
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
Assay:
≥99.7%
Grade:
Hybri-Max™
Technique(s):
cell culture | hybridoma: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)
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grade

Hybri-Max™

Quality Level

400

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

feature

subscription eligible

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

impurities

endotoxin, tested, ≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

This product features a liner in the cap designed to prevent leakage. In rare cases, the liner may remain on the neck of the bottle after cap removal. This does not affect the product′s integrity. If this occurs, simply reattach the liner to the cap.
100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

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Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
118K2300
118K2323
118K2305
118K2304
108K2336

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Protocols

诱导多能干细胞重编程实验方案

产生人诱导多能干细胞（iPSC）的干细胞重编程实验方案，包括基于病毒和非病毒RNA的方法。

间充质干细胞培养方案

有关间充质的信息，特别是间充质干细胞培养实验方案用于间充质干细胞（MSC）分离、扩增和分化的分步细胞培养方案。

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

View All Protocols

Articles

良好的细胞库操作实践

用于细胞系冷冻保存的良好细胞库操作实践概述。

新型无异种和无血清成骨分化培养基中人间充质干细胞的加速骨细胞分化

新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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