DUO92102
Duolink® In Situ Orange Starter Kit Mouse/Rabbit
Synonym(s):
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
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About This Item
product line
Duolink®
technique(s)
proximity ligation assay: suitable
fluorescence
λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
suitability
suitable for fluorescence
storage temp.
−20°C
Application
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
The Duolink® In Situ Orange Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Orange fluorescence detection reagents are often used with Cyanine 3 filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Kit Components Also Available Separately
- Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)
signalword
Danger
wgk
WGK 3
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
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Protocols
本页详细介绍了Duolink®原位精简实验方案
Articles
本实验方案描述了如何对细胞和组织样品中的蛋白质进行免疫荧光检测。
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
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蛋白质与核酸相互作用试剂，以及用于研究蛋白质-RNA、蛋白质-DNA、蛋白质-蛋白质相互作用和相关应用的资源。
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
利用邻位连接技术对蛋白质互作、翻译后修饰和低表达蛋白进行检测、定量和成像应用。