EMS0001
PNGase Fast
recombinant, expressed in E. coli
Synonym(s):
N-Glycosidase F, PNGase F, Peptide N-glycosidase
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About This Item
UNSPSC Code:
41131616
Form:
ready-to-use solution
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
conjugate
(N-linked)
grade
Proteomics Grade
form
ready-to-use solution
shipped in
wet ice
storage temp.
2-8°C
General description
Peptide-N-glycosidase F (PNGase F) belongs to an enzyme family, that are mainly used for the deglycosylation of N-linked glycans.
Application
PNGase Fast may be used to immobilize in order to perform deglycosylation. It may also be used to immobilize onto methacrylate based monolithic support to release the N-linked carbohydrate moieties from glycoproteins.
Biochem/physiol Actions
Peptide-N-glycosidase F (PNGase F) cleaves asparagine-linked high mannose, hybrid and complex oligosaccharides from glycoproteins. It can also deaminate the asparagine to aspartic acid. PNGase Fast enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, to be prepared for downstream chromatography or mass spectrometry analysis. PNGase Fast creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
Storage Class
10 - Combustible liquids
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