LYSC9000
Lys-C Protease
Cleaves at lysine residues, suitable for mass spectrometry, from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa)
Synonym(s):
Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
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Product Name
Lys-c (lysyl-endopeptidase), Active, from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His Tag, solution
biological source
Achromobacter lyticus
recombinant
expressed in E. coli (206-473aa)
grade
Proteomics Grade
form
ready-to-use solution
concentration
1 mg/mL
optimum pH
8.5
pH range
9.0-9.5(catalytic)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
General description
Recombinant His tag Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.
Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.
Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.
Application
The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.
Biochem/physiol Actions
This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.
Packaging
1mg/ml in Plastic
Preparation Note
Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.
Store product at -20°C for up to one year. Avoid freeze/thaw cycles.
Other Notes
For R&D only.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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