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Merck

M1028

Magnesium chloride solution

Molecular Biology, 1.00 M±0.01 M

Synonym(s):

MgCl2, dichloromagnesium, magnesium chloride

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About This Item

Linear Formula:
MgCl2
CAS Number:
7786-30-3
Molecular Weight:
95.21
NACRES:
NA.52
PubChem Substance ID:
24896582
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Grade:
Molecular Biology
Form:
liquid
Solubility:
water: soluble

Key Documents

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InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

grade

Molecular Biology

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

technique(s)

DNA sequencing: suitable

impurities

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for electrophoresis, suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, NICKase and protease, none detected

Quality Level

300

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General description

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Features and Benefits

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL4409
WXBD6598V
SLCL2579
SLCK1344
SLCK0066

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Antimo Migliaccio et al.
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Bert De Rybel et al.
Science (New York, N.Y.), 345(6197), 1255215-1255215 (2014-08-12)
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Exome sequencing studies in complex diseases are challenged by the allelic heterogeneity, large number and modest effect sizes of associated variants on disease risk and the presence of large numbers of neutral variants, even in phenotypically relevant genes. Isolated populations
Hideyuki Arita et al.
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Protocols

二乙醇胺测定法用于碱性磷酸酶的酶法测定（EC 3.1.3.1）

碱性磷酸酶的酶法测定，二乙醇胺法（EC 3.1.3.1)

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

己糖激酶的酶促检测

在测定己糖激酶活性时，使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。

变溶菌素的酶促检测

在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

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