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N7653

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonym(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
4264-83-9
Molecular Weight:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
24897835
NACRES:
NA.83
MDL number:
MFCD00007319

Key Documents

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Product Name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Related Categories

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year when stored below 0 °C.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

Storage Class

12 - Non Combustible Liquids

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L Jonasson et al.
Scientific reports, 7(1), 17545-17545 (2017-12-14)
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European journal of medicinal chemistry, 178, 108-115 (2019-06-09)
As dual regulators, the PTP-1B signaling pathway and α-glucosidase slow glucose release and increase the degree of insulin sensitivity, representing a promising therapeutic target for type 2 diabetes. In this study, we systematically examined the in vivo and in vitro anti-diabetic activities
Cheng-Chi Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11661-11666 (2008-08-12)
Cancer-associated carbohydrate antigens are often found on the surface of cancer cells. Understanding their roles in cancer progression will lead to the development of new therapeutics and high-sensitivity diagnostics for cancers. Globo H is a member of this family, which
I K Manoylov et al.
Clinical and experimental immunology, 197(3), 329-340 (2019-04-23)
Type 1 diabetes mellitus is an autoimmune metabolic disorder characterized by chronic hyperglycemia, the presence of autoreactive T and B cells and autoantibodies against self-antigens. A membrane-bound enzyme on the pancreatic beta-cells, glutamic acid decarboxylase 65 (GAD65), is one of
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Kamarajan, et al.
International journal of biomedical research, 80(8), 467-474 (2007)
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