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13024AST

Astec® CHIROBIOTIC® R Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Chiral Recognition HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
ristocetin A glycopeptide phase
Pore size:
100 Å
Matrix:
high-purity silica gel particle platform, fully porous particle
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Product Name

Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Segment

100

description

HPLC column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature, 241 bar pressure (3500 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

matrix

high-purity silica gel particle platform, fully porous particle

matrix active group

ristocetin A glycopeptide phase

particle size

5 μm

pore size

100 Å

operating pH range

3.5-6.8

separation technique

chiral

General description

CHIROBIOTIC® R, based on the ristocetin A glycopeptide covalently bonded to high purity silica particles, has shown particular applicability to enantiomers of anionic compounds. Selectivity on CHIROBIOTIC® R strongly correlates to the organic modifier, favoring the alcohol-type mobile phases by a large margin.

  • Bonded phase: Ristocetin A
  • Operating pH range: 3.5 - 6.8
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application


  • Sensitive Determination of Lactic Acid Isomers: The Astec® CHIROBIOTIC® R Chiral HPLC Column was crucial in a study by Henry H et al., where it facilitated the sensitive determination of D-lactic acid and L-lactic acid in urine using high-performance liquid chromatography-tandem mass spectrometry. This method is pivotal for clinical diagnostic applications, showcasing the column′s ability to resolve similar compounds that are challenging to differentiate by other means (Henry H et al., 2012).


  • Enantioseparation of Cyclopentanecarboxylic and Cyclohexanecarboxylic Acids: The column was also utilized by Berkecz R et al. to achieve high-performance liquid chromatographic enantioseparation of complex 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids. This application underscores the column′s versatility and efficacy in separating closely related chiral acids, essential for biochemical research and pharmaceutical product development (Berkecz R et al., 2009).

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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