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D7008

Dihydroethidium

Name: Dihydroethidium
Brand: SIAL

≥95% purity, powder

Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₂₁N₃

CAS Number:

104821-25-2

Molecular Weight:

315.41

NACRES:

NA.47

PubChem Substance ID:

24894069

UNSPSC Code:

12171500

MDL number:

MFCD00077335

Beilstein/REAXYS Number:

5107482

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Properties

Product Name

Dihydroethidium, ≥95%

Quality Level

100

assay

≥95%

form

powder

technique(s)

titration: suitable

color

pink to very dark red

solubility

chloroform: 20 mg/mL

fluorescence

λ_ex 370 nm; λ_em 420 nm (for cytoplasm of living cells), λ_ex 535 nm; λ_em 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14

InChI

1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3

InChI key

XYJODUBPWNZLML-UHFFFAOYSA-N

Description

Application

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Packaged under Argon.

Other Notes

B-ring reduced analog of ethidium

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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