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Merck

N7660

4-Nitrophenol

chromogenic, liquid

Synonym(s):

p-Nitrophenol solution

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About This Item

Empirical Formula (Hill Notation):
C6H5NO3
CAS Number:
Molecular Weight:
139.11
UNSPSC Code:
12352204
eCl@ss:
39032018
PubChem Substance ID:
NACRES:
NA.28
MDL number:
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Product Name

4-Nitrophenol solution, 10 mM

form

liquid

concentration

10 mM

storage temp.

2-8°C

SMILES string

Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H5NO3/c8-6-3-1-5(2-4-6)7(9)10/h1-4,8H

InChI key

BTJIUGUIPKRLHP-UHFFFAOYSA-N

Gene Information

human ... UGT1A4(54657)

General description

4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of paracetamol. 4-Nitrophenol is a chromogenic catalytic by product of the synthetic substrate 4-nitrophenyl phosphate. It is majorly used as a reference standard in alkaline phosphatase assay. 4-Nitrophenol is nonbiodegradable and its derivatives are used for herbicide and fungicide production.

Application

4-Nitrophenol solution has been used:
  • in the generation of standard curve for quantifying alkaline phosphatase activity of mesenchymal stem cells
  • bone marrow stromal cells (BMSCs)
  • for quantifying β-hexosaminidase activity in HeLa cells
  • as a standard for calorimetric determination of alkaline phosphatase activity at 405 nm
  • for assessing the β-hexosamindase activity

Preparation Note

Prepared as a 1.39 mg/ml solution suitable for use as a calibrator in alkaline phosphatase assays (such as Bessey-Lowry-Brock method) using p-nitrophenol phosphate as a substrate.


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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Storage Class

12 - Non Combustible Liquids



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