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03-0163-00

SMC® Human TNFα High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human TNFα in human serum and plasma

Synonym(s):

TNF-a immunoassay kit, TNF-alpha Single Molecule Counting Kit, TNF-alpha immunoassay kit, TNF-alpha ultra-sensitive immunoassay, Tumor necrosis factor α

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About This Item

NACRES:
NA.77
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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Product Name

SMC® Human TNFα High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human TNFα in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

P01375

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine primarily produced by activated macrophages and T cells, playing a central role in the immune response. It is crucial for the regulation of inflammation, apoptosis, and the activation of other immune cells, facilitating the body′s defense against infections and tumors. However, excessive or dysregulated TNF-α production is associated with various inflammatory and autoimmune diseases, such as rheumatoid arthritis and Crohn′s disease, making it a significant target for therapeutic interventions.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
  • Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
  • Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
  • Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 50-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 113% Plasma: 105%
  • Recovery: Serum: 95% Plasma: 83%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although TNFα was detectable in non-human primate, rat, feline, canine and equine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B

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