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203390

(±)-Blebbistatin

≥97% (HPLC), solid, Myosin II inhibitor, Calbiochem®

Synonym(s):

(±)-Blebbistatin

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About This Item

Empirical Formula (Hill Notation):
C18H16N2O2
CAS Number:
674289-55-5
Molecular Weight:
292.33
MDL number:
MFCD04113775
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥97% (HPLC)
Form:
solid
Storage condition:
OK to freeze, protect from light
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Product Name

(±)-Blebbistatin, (±)-Blebbistatin, CAS 674289-55-5, is a cell-permeable, selective, and reversible inhibitor of nonmuscle myosin II. Blocks cell blebbing and disrupts cell migration & cytoKinesis in vertebrate cells.

Quality Segment

100

assay

≥97% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

yellow

solubility

methanol: 1.5 mg/mL, 100% DMSO: 100 mg/mL, 90% DMSO: 75 mg/mL

shipped in

wet ice

storage temp.

−20°C

SMILES string

N2(CCC3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1

InChI

1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3

InChI key

LZAXPYOBKSJSEX-UHFFFAOYSA-N

General description

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389 ) in 90% DMSO is also available.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
ATPase
Product does not compete with ATP.
Reversible: yes
Target IC50: <100 µM inhibiting the ATPase and gliding motility of human platelets

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Other Notes

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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