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Merck

S7907

Sodium phosphate dibasic

BioXtra, ≥99.0%

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
PubChem Substance ID:
eCl@ss:
38070211
UNSPSC Code:
12161700
NACRES:
NA.25
EC Number:
231-448-7
MDL number:
Technical Service
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product line

BioXtra

Quality Level

assay

≥99.0%

form

powder or crystals

impurities

≤0.1% Insoluble matter

pH

8.0-11.0 (20 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.05%, sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.002%, Cu: ≤0.0005%, Fe: ≤0.002%, K: ≤0.005%, Mg: ≤0.005%, NH4+: ≤0.05%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

<0.05 at 260 in H2O at 1 M, <0.05 at 280 in H2O at 1 M

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

General description

Sodium phosphate dibasic (NaH2PO4), also known as disodium phosphate, is a commonly used phosphate salt in biochemistry research due to its versatile properties. It is a key component in the preparation of phosphate buffer saline (PBS) and phosphate buffer, a commonly used buffer solution in many biological applications. In addition to its buffer properties, NaH2PO4 is also used as a precipitating agent to form silver orthophosphate (Ag3PO4) powders, making it a valuable tool in many laboratory settings. Disodium phosphate is also a widely used emulsifier in dairy products such as cheese and other foods.

Application

Sodium phosphate dibasic has been used:
  • as a component of phosphate buffered saline (PBS)
  • as a component of buffer for the preparation of cryopreservation diluent
  • in the preparation of McIlvaine buffer (citrate-phosphate buffer) for toluidine blue staining
  • in the preparation of the S9 mix for the Ames test, and SOS Chromotest
  • in the preparation of calcium deficient hydroxyapatite (CDHA) paste during the fabrication process of the CDHA scaffold

Features and Benefits

  • The BioXtra product line ensures high-quality products for consistent results.
  • High level of assay ( ≥99.0%), indicating its purity.
  • Powder form for easy handling and use.


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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