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56741

IGEPAL® CA-630

Synonym(s):

Nonidet™ P 40, Nonylphenyl-polyethylenglycol, Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
MDL number:
MFCD00214058
UNSPSC Code:
12161902
NACRES:
NA.25
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description

non-ionic

Quality Segment

100

form

liquid

mol wt

~603.0 g/mol

refractive index

n20/D 1.492

CMC

0.08 mM (20-25°C)

transition temp

cloud point 63-67  °C

density

1.06 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤1000 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤1000 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

HLB

13

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
  • In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S
  • As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
  • In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
  • As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Analysis Note

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Other Notes

Nonionic, non-denaturing detergent

Legal Information

Nonidet is a trademark of Shell Chemical Co.
IGEPAL is a registered trademark of Syensqo

Disclaimer

May become turbid on storage; a clear product can be obtained on heating to 40°C.
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

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Danger

Storage Class

10 - Combustible liquids

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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