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A9400

7-Aminoactinomycin D

Name: 7-Aminoactinomycin D
Brand: SIGMA

~97% (HPLC), powder, fluorescent DNA stain

Synonym(s):

7-AAD

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About This Item

Empirical Formula (Hill Notation):

C₆₂H₈₇N₁₃O₁₆

CAS Number:

7240-37-1

Molecular Weight:

1270.43

NACRES:

NA.77

PubChem Substance ID:

24891445

UNSPSC Code:

41106305

MDL number:

MFCD00077295

Beilstein/REAXYS Number:

5915844

Assay:

~97% (HPLC)

Form:

powder

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Properties

Product Name

7-Aminoactinomycin D, ~97% (HPLC), powder

assay

~97% (HPLC)

Quality Segment

300

form

powder

color

red to very dark purple

antibiotic activity spectrum

neoplastics

mode of action

DNA synthesis | interferes

storage temp.

2-8°C

SMILES string

CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2cc(N)c(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O

InChI

1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)/t32-,33-,36+,37+,42-,43-,44+,45+,48+,49?/m1/s1

InChI key

YXHLJMWYDTXDHS-SGILFZQNSA-N

Description

General description

7-Aminoactinomycin D (7-ADD), an analog of actinomycin D (AD) is a membrane-impermeable red fluorescent DNA dye. It can selectively bind GC regions of DNA. 7-ADD is used in flow cytometry to differentiate between viable, apoptotic, and late apoptotic/dead cells. This DNA stain finds its application in chromosome analysis, cell cycle studies, and quantification of apoptosis.

Chemical structure: peptide

Application

7-Aminoactinomycin D has been used:

for nuclei visualization in mouse tail skin
as a nuclear stain in imaging flow cytometry for nucleus-protein colocalization
in flow cytometry to determine the level of CD16 (Fc gamma III receptor) and CD32 (Fc gamma II receptor) in epidermal cells

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

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pictograms

GHS06

signalword

Danger

hcodes

H300

pcodes

P301 + P330 + P331 + P310

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c