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Merck

G5160

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
EC Number:
Specific activity:
≥8.0 units/mg solid
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form

solid

Quality Segment

specific activity

≥8.0 units/mg solid

contains

dextrin as extender

storage temp.

−20°C

General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:
  • in in vitro nuclear magnet resonance (NMR)
  • as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
  • to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
  • for enzymatic hydrolysis of lactose
  • for reversed-phase (RP) adsorption
  • in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Other Notes

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1



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