U0508
Monoclonal Anti-Ubiquitin antibody produced in mouse
clone 6C1, ascites fluid
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About This Item
Conjugate:
unconjugated
Clone:
6C1, monoclonal
Application:
ARR, ELISA (i), WB
Citations:
29
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6C1, monoclonal
contains
15 mM sodium azide
species reactivity
human, rat, mouse, bovine
technique(s)
indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)
General description
Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.
Immunogen
purified bovine ubiquitin conjugated to KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yu Wang et al.
Autophagy, 11(11), 2033-2047 (2015-12-10)
Autophagy plays critical roles in plant responses to stress. In contrast to the wealth of information concerning the core process of plant autophagosome assembly, our understanding of the regulation of autophagy is limited. In this study, we demonstrated that transcription
Kalidou Ndiaye et al.
Journal of ovarian research, 8, 12-12 (2015-04-18)
LAPTM4B is a member of the lysosome-associated transmembrane protein superfamily that is differentially expressed in normal human tissues and upregulated in various types of carcinomas. These proteins are thought to be involved in the regulation of cell proliferation and survival.
Clinton Yu et al.
Nature communications, 6, 10053-10053 (2015-12-04)
The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby