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05-1307-AF488 Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate

This Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.

Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: UBB UniProt Number: P0CG47
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05-1307-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICCRbAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-1307-AF488
Description Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate
Alternate Names
  • Polyubiquitin-B
  • Ubiquitin, Lys48-Specific
  • Ubiquitin
Background InformationPolyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality SegmentMQ100
Applications
ApplicationThis Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.
Biological Information
ImmunogenImmunogen used was human di-ubiquitin linked at Lys48.
EpitopeLys48 ubiquitination site
CloneApu2
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rabbit based on 100% sequence homology. Based on sequence homology, broad species cross-reactivity is expected. .
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBB
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
05-1307-AF488 04055977298833

Documentation

Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate Certificates of Analysis

TitleLot Number
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate 2992676
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate - 3524853 3524853
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate -2844419 2844419
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate -Q2610673 Q2610673
Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate_2840392 2840392

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

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Title
Post-Translational Modifications