126579
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
Synonym(s):
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free
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About This Item
Assay:
≥95% (Albumin, electrophoresis)
Form:
lyophilized powder
Technique(s):
immunofluorescence: suitable
Impurities:
≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture
description
Merck USA index - 14, 8468
Quality Level
assay
≥95% (Albumin, electrophoresis)
form
lyophilized powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
immunofluorescence: suitable
impurities
≤0.2% fatty acids, ≤3.0% Ash, ≤5% moisture
color
pale green
solubility
aqueous buffer: 20 mg/mL, water: 20 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Preparation Note
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Material may take up to 24 h to fully dissolve.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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