AB5300
Anti-Amyloid Precursor Protein (APP) Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
APP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, monkey, mouse
Application:
IP, WB
Citations:
5
Product Name
Anti-Amyloid Precursor Protein Antibody, universal, serum, Chemicon®
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, monkey, mouse
should not react with
bovine
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Immunogen
APP (aa 99-126) conjugated to KLH.
Epitope: universal
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, universal is validated for use in IP, WB for the detection of Amyloid Precursor Protein.
Western blot: 1:250 - 1:1,000: need to reduce and alkylate disulfide bonds of APP to expose the epitope on APP transferred to nitrocellulose or PVDF prior to immunoblotting.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Need to reduce and alkylate disulfide bonds to expose the epitope
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Amyloid Precursor Protein (APP), Universal. Specific to amino acids 99-126.
Physical form
Serum. Liquid in PBS with 0.01% azide.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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