Polyvinylpyrrolidone

Polyvinylpyrrolidone (PVP) can form complexes with phenolics and alkaloids. The complex formation allows removal of phenolics and alkaloids from plant samples, thereby inhibiting any modification of proteins by them and any hindrance they may cause in spectrophotometric determinations of protein content. PVP might also enhances the stability of enzymes.

Polyvinylpyrrolidone (PVP), a multifunctional synthetic polymer, finds utility in life science and biochemical research. This water-soluble polymer is versatile, serving as a stabilizing agent, emulsifier, suspending agent, solubilizing agent, protective colloid, and complexing agent. Its protective colloid properties are particularly valuable in stabilizing proteins, enzymes, and other biological substances.

Polyvinylpyrrolidone has been used in the purification of glutamine synthetases from plant samples. It has been used to block the PVDF (polyvinylidene difluoride) membrane and to avoid nonspecific binding.

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Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.