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E1769

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

Name: <I>N</I>-(3-Dimethylaminopropyl)-<I>N</I>′-ethylcarbodiimide hydrochloride
Brand: SIAL

BioXtra

Synonym(s):

N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC, EDC hydrochloride, WSC hydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₇N₃ · HCl

CAS Number:

25952-53-8

Molecular Weight:

191.70

NACRES:

NA.25

PubChem Substance ID:

24894433

UNSPSC Code:

12161700

EC Number:

247-361-2

MDL number:

MFCD00012503

Beilstein/REAXYS Number:

5764110

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Properties

Product Name

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, BioXtra

product line

BioXtra

Quality Level

200

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

≤0.1%

mp

110-115 °C (lit.)

solubility

H₂O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow

anion traces

sulfate (SO₄^2-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

storage temp.

−20°C

SMILES string

Cl.CCN=C=NCCCN(C)C

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

InChI key

FPQQSJJWHUJYPU-UHFFFAOYSA-N

Description

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:

for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
for cross-linking of nanofiber matrices with mouse lung extract
for activation of substrate XNA oligonucleotides

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.