87748
3,3′,5,5′-Tetramethylbenzidine
TMB substrate, chromogenic, ≥98.0% (NT), powder
Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
Beilstein/REAXYS Number:
2808541
Product Name
3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)
Quality Level
assay
≥98.0% (NT)
form
powder
technique(s)
UV/Vis spectroscopy: suitable
mp
166-171 °C, 168-171 °C (lit.)
storage temp.
2-8°C
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
- enzyme-immunoassay (EIA)
- indirect competitive enzyme-linked immunosorbent assay (ELISA)
- sandwich enzyme-linked immunosorbent assay
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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