C1655
Cholera Toxin B subunit
GM1-binding, lyophilized powder
Synonym(s):
CTB
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About This Item
Form:
lyophilized powder
Quality level:
Product Name
Cholera Toxin B subunit, FITC conjugate, lyophilized powder
conjugate
FITC conjugate
Quality Level
form
lyophilized powder
mol wt
~12 kDa
composition
Protein, ~20% Lowry
extent of labeling
~1.0 mol FITC per mol protein
storage temp.
2-8°C
SMILES string
CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
InChI
1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChI key
YDXZSNHARVUYNM-UHFFFAOYSA-N
Application
Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
- to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat
- to stain epididymal sperms of mice
- to stain peritoneal macrophages of mice
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Analysis Note
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Emily Igel et al.
The Journal of biological chemistry, 297(3), 101106-101106 (2021-08-24)
Polymorphisms in the apolipoprotein E (apoE) gene are risk factors for chronic inflammatory diseases including atherosclerosis. The gene product apoE is synthesized in many cell types and has both lipid transport-dependent and lipid transport-independent functions. Previous studies have shown that
Almut Dufner et al.
Cell communication and signaling : CCS, 9(1), 6-6 (2011-03-15)
The B cell antigen receptor (BCR) and pathogen recognition receptors, such as Toll-like receptor 4 (TLR4), act in concert to control adaptive B cell responses. However, little is known about the signaling pathways that integrate BCR activation with intrinsic TLR4
Birgit Bauer et al.
FEBS letters, 541(1-3), 155-162 (2003-04-23)
Protein kinase (PK) Ctheta and Akt/PKBalpha cooperate in T cell receptor/CD28-induced T cell signaling. We here demonstrate the recruitment of endogenous Akt1 and PKCtheta to lipid rafts in CD3-stimulated T cells. Further we show that Myr-PKCtheta mediates translocation of endogenous