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F7378

Fluorescein diacetate

Name: Fluorescein diacetate
Brand: SIGMA

Powder

Synonym(s):

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₁₆O₇

CAS Number:

596-09-8

Molecular Weight:

416.38

NACRES:

NA.47

PubChem Substance ID:

24894939

UNSPSC Code:

12171500

EC Number:

209-877-6

MDL number:

MFCD00005062

Beilstein/REAXYS Number:

62575

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Properties

Product Name

Fluorescein diacetate, used as cell viability stain

Quality Level

200

form

powder

technique(s)

titration: suitable

color

white to faint yellow

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

Description

General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.

Lipase substrate

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Biomedical Materials (Bristol, England), 9, 035011-035011 (2014)

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