F9037
Formamide
≥99.5% (GC), BioReagent, Molecular Biology
Synonym(s):
Amide C1, Formic amide
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About This Item
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
UNSPSC Code:
12352111
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-842-0
Beilstein/REAXYS Number:
505995
MDL number:
Assay:
≥99.5% (GC)
Grade:
Molecular Biology
Technique(s):
electrophoresis: suitable, immunohistochemistry: suitable
Bp:
210 °C (lit.)
Vapor pressure:
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
grade
Molecular Biology
Quality Level
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
product line
BioReagent
assay
≥99.5% (GC)
form
liquid
autoignition temp.
932 °F
technique(s)
electrophoresis: suitable, immunohistochemistry: suitable
refractive index
n20/D 1.447 (lit.)
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
density
1.134 g/mL at 25 °C (lit.)
suitability
suitable for nucleic acid hybridization
storage temp.
2-8°C
SMILES string
NC=O
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
General description
Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.
Application
- nucleic acid hybridization
- cryoprotectant and gel-stabilizer
- destabilize nucleic acid duplexes
- elimination of secondary structure of nucleic acids
- preparation of hybridization buffers for RNA and DNA application
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Other applications include:
Other applications include:
- Preparation of brain sections of mice for BrdU immunohistochemistry
- FISH analysis of HeLa cells.
- Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).
Features and Benefits
- Deionized for molecular biology applications
- Packaged under argon gas in amber bottles to protect from oxidation
- Used for DNA renaturation or DNA-RNA hybridization
- Reduces thermal stability of double stranded nucleic acids
Other Notes
The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.
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signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
305.6 °F
flash_point_c
152 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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