A8456
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride
≥97.0% (HPLC)
Synonym(s):
AEBSF
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
Empirical Formula (Hill Notation):
C8H10FNO2S · HCl
CAS Number:
Molecular Weight:
239.69
UNSPSC Code:
12352202
NACRES:
NA.77
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7604627
InChI
1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
SMILES string
FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
InChI key
WRDABNWSWOHGMS-UHFFFAOYSA-N
biological source
synthetic (organic)
assay
≥97.0% (HPLC)
form
powder
Quality Level
solubility
H2O: 50 mg/mL
storage temp.
−20°C
General description
stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.
Application
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
- as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
- as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
- as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates
Recommended concentrations for use are 0.1-1 mM.
Biochem/physiol Actions
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.
AEBSF can inhibit acetylcholinesterase (AChE).
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ilario Mennella et al.
The Journal of nutrition, 145(9), 2169-2175 (2015-07-17)
Food palatability increases food intake and may lead to overeating. The mechanisms behind this observation are still largely unknown. The aims of this study were the following: 1) to elucidate the plasma responses of endocannabinoids, N-acylethanolamines, and gastrointestinal peptides to
Ignacio Pedrós et al.
Biochimica et biophysica acta, 1842(9), 1556-1566 (2014-06-03)
The present study had focused on the behavioral phenotype and gene expression profile of molecules related to insulin receptor signaling in the hippocampus of 3 and 6 month-old APPswe/PS1dE9 (APP/PS1) transgenic mouse model of Alzheimer's disease (AD). Elevated levels of
Sara Lind Jepsen et al.
The Journal of clinical endocrinology and metabolism, 105(1) (2019-10-15)
The gastrointestinal hormone ghrelin stimulates growth hormone secretion and appetite, but recent studies indicate that ghrelin also stimulates the secretion of the appetite-inhibiting and insulinotropic hormone glucagon-like peptide-1 (GLP-1). To investigate the putative effect of ghrelin on GLP-1 secretion in
Niina Aaltonen et al.
Biological procedures online, 22, 6-6 (2020-03-20)
Serine hydrolases (SHs) are a functionally diverse family of enzymes playing pivotal roles in health and disease and have emerged as important therapeutic targets in many clinical conditions. Activity-based protein profiling (ABPP) using fluorophosphonate (FP) probes has been a powerful
Robert J Snelgrove et al.
The Journal of allergy and clinical immunology, 134(3), 583-592 (2014-03-19)
The fungal allergen Alternaria alternata is implicated in severe asthma and rapid onset life-threatening exacerbations of disease. However, the mechanisms that underlie this severe pathogenicity remain unclear. We sought to investigate the mechanism whereby Alternaria was capable of initiating severe
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service