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46393

Fluo-3

suitable for fluorescence, ~70%

Synonym(s):

4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid

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About This Item

Empirical Formula (Hill Notation):
C36H30Cl2N2O13
CAS Number:
123632-39-3
Molecular Weight:
769.54
NACRES:
NA.32
PubChem Substance ID:
329757162
UNSPSC Code:
12352106
MDL number:
MFCD00083337
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assay

~70%

Quality Level

100

form

solid

solubility

H2O: soluble

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

InChI

1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

InChI key

OZLGRUXZXMRXGP-UHFFFAOYSA-N

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Susanne Staehlke et al.
Cell & bioscience, 8, 22-22 (2018-03-29)
Biomaterial modifications-chemical and topographical-are of particular importance for the integration of materials in biosystems. Cells are known to sense these biomaterial characteristics, but it has remained unclear which physiological processes bio modifications trigger. Hence, the question arises of whether the
P A Vandenberghe et al.
Journal of immunological methods, 127(2), 197-205 (1990-03-09)
Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3
Qijin Zhao et al.
Cell death and differentiation, 26(9), 1600-1614 (2018-11-06)
Mitochondrial superoxide dismutase 2 (SOD2) is a major antioxidant defense enzyme. Here we provide evidence that SOD2 plays critical roles in maintaining calcium homeostasis in newly generated embryonic cerebral cortical neurons, which is essential for normal mitochondrial function and subcellular
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