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46951

Fluorescein isothiocyanate isomer I

≥97.5% (HPLC)

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
EC Number:
222-042-0
UNSPSC Code:
12171500
PubChem Substance ID:
329757274
Beilstein/REAXYS Number:
1407295
MDL number:
MFCD00005063
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Quality Level

100

assay

≥97.5% (HPLC)

form

powder

impurities

≤1.5% Isomer II

mp

>360 °C (lit.)

solubility

acetone: soluble 0.1 g/10 mL

fluorescence

λex 492 nm; λem 518 nm (green), λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

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pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

hcodes

H317,H334

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Wenhuo Hu et al.
Blood, 125(14), 2206-2216 (2015-01-31)
Hematopoietic stem cells (HSCs) possess the ability to generate all hematopoietic cell types and to self-renew over long periods, but the mechanisms that regulate their unique properties are incompletely understood. Herein, we show that homozygous deletion of the miR-29a/b-1 bicistron
Aram Ghalali et al.
Carcinogenesis, 35(7), 1547-1555 (2014-01-24)
Epidemiological studies indicate that statins, cholesterol-lowering drugs, prevent aggressive prostate cancer and other types of cancer. Employing essentially non-prostate cell lines, we previously showed that statins rapidly downregulate nuclear levels of phosphorylated Akt via P2X7, a purinergic receptor recently implicated
Marina Vivarelli et al.
Kidney international, 87(3), 602-609 (2015-01-08)
Alloimmune antenatal membranous nephropathy (MN) during pregnancy results from antibodies produced by a neutral endopeptidase (NEP)-deficient mother. Here we report two recent cases that provide clues to the severity of renal disease. Mothers of the two children had circulating antibodies
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